真核肽链释放因子1抗体应用方法

中文名称    真核肽链释放因子1抗体    

英文名称    eRF1    

别    名    Cl1 protein; D5S1995; ERF; eRF1; ERF1_HUMAN; ETF1; Eukaryotic peptide chain release factor subunit 1; Eukaryotic release factor 1; Eukaryotic translation termination factor 1; MGC111066; Polypeptide chain release factor 1; Protein Cl1; RF1; Sup45 (yeast omnipotent suppressor 45) homolog like 1; SUP45L1; TB3 1; TB3-1.    

供 应 商    远慕生物      

研究领域    细胞生物  转录调节因子  表观遗传学  G蛋白信号      

抗体来源    Rabbit    

克隆类型    Polyclonal    

交叉反应    Human, Mouse, Rat, Dog, Cow, Horse, Zebrafish, Arabidopsis Thaliana, Xenopus laevis    

产品应用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 真核肽链释放因子1抗体（石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.    

分 子 量    49kDa    

细胞定位    细胞浆     

性    状    Lyophilized or Liquid    

浓    度    1mg/1ml    

免 疫 原    KLH conjugated synthetic peptide derived from human eRF1    

亚    型    IgG    

纯化方法    affinity purified by Protein A    

储 存 液    Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4    

保存条件    Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.    

真核肽链释放因子1抗体产品介绍    background:

This gene encodes a class-1 polypeptide chain release factor. The encoded protein plays an essential role in directing termination of mRNA translation from the termination codons UAA, UAG and UGA. This protein is a component of the SURF complex which promotes degradation of prematurely terminated mRNAs via the mechanism of nonsense-mediated mRNA decay (NMD). Alternate splicing results in coding and non-coding transcript variants. Pseudogenes of this gene are found on chromosomes 5, 7 and X. [provided by RefSeq, Jan 2012].

Function:
Directs the termination of nascent peptide synthesis (translation) in response to the termination codons UAA, UAG and UGA. Component of the transient SURF complex which recruits UPF1 to stalled ribosomes in the context of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons.

Subunit:
Heterodimer of two subunits, one of which binds GTP. Component of the transient SURF (SMG1-UPF1-eRF1-eRF3) complex.

Subcellular Location:
Cytoplasm.

Similarity:
Belongs to the eukaryotic release factor 1 family.