内质网蛋白ERp72抗体说明书

中文名称    内质网蛋白ERp72抗体    

英文名称    ERp72    

别    名    Calcium binding protein intestinal related; Endoplasmic reticulum resident protein 70; Endoplasmic reticulum resident protein 72; ER protein 70; ER protein 72; ERP 70; ERp 72; ERp-72; ERP70; ERp72; PDIA 4; PDIA4; PDIA4_HUMAN; PDIR; Protein disulfide isomerase A4; Protein disulfide isomerase associated 4; Protein disulfide isomerase family A member 4; Protein disulfide isomerase related protein; Protein disulfide-isomerase A4; Protein ERp 72; Protein ERp72.    

供 应 商    远慕生物

研究领域    肿瘤  细胞生物  信号转导      

抗体来源    Rabbit    

克隆类型    Polyclonal    

交叉反应    Human, Mouse, Rat, Cow, Rabbit, Sheep, Monkey,     

产品应用    WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 内质网蛋白ERp72抗体（石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.    

分 子 量    71kDa    

细胞定位    细胞浆     

性    状    Lyophilized or Liquid    

浓    度    1mg/1ml    

免 疫 原    KLH conjugated synthetic peptide derived from human ERp72    

亚    型    IgG    

纯化方法    affinity purified by Protein A    

储 存 液    Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4    

保存条件    Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.    

内质网蛋白ERp72抗体产品介绍    background:

Mammals defend themselves against intracellular pathogens through presentation of cytoplasmically derived short pathogenic peptides to the cell surface of cytotoxic T lymphocytes, which subsequently leads to cytotoxic events with respect to the affected cell. Antigen presentation is mediated by major histocompatibility complex (MHC) class I molecules, which bind and coordinate short pathogenic peptides. The proper folding and assembly of MHC class I molecules in the endoplasmic reticulum (ER) involve a number of components. MHC class I molecules assemble in the ER with chaperones before binding to the transporter associated with antigen processing (TAP) protein. ERp57 is a component of the MHC class I pathway that appears to interact with MHC class I molecules before they associate with TAP. ERp72, also designated protein disulfide-isomerase A4, is involved in the catalysis of protein -S-S- bond rearrangement. ERp57 and ERp72 may act as proteases, protein disulfide isomerases, phospholipases or a combination of these.

Subunit:
Part of a large chaperone multiprotein complex comprising DNAJB11, HSP90B1, HSPA5, HYOU, PDIA2, PDIA4, PDIA6, PPIB, SDF2L1, UGT1A1 and very small amounts of ERP29, but not, or at very low levels, CALR nor CANX.

Subcellular Location:
Endoplasmic reticulum lumen. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

Similarity:
Belongs to the protein disulfide isomerase family.
Contains 3 thioredoxin domains.

Database links:

Entrez Gene: 415110 Cow

Entrez Gene: 9601 Human

Entrez Gene: 12304 Mouse

Entrez Gene: 116598 Rat

SwissProt: Q29RV1 Cow

SwissProt: P13667 Human

SwissProt: P08003 Mouse

SwissProt: P38659 Rat

Unigene: 93659 Human

Unigene: 2442 Mouse

Unigene: 39305 Rat


Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.